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αil-4 antibody  (Bio X Cell)


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    Bio X Cell αil-4 antibody
    αil 4 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/αil-4 antibody/product/Bio X Cell
    Average 90 stars, based on 1 article reviews
    αil-4 antibody - by Bioz Stars, 2026-05
    90/100 stars

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    Fixation method influenced the detection in i NKT cells of several cytokines. (A – C) C57BL/6 mice were either mock treated or injected i.v. with 1μg αGalCer and 90 min later the expression of the cytokines IL-4 (clone BVD6-24G2 or 11B11, as indicated in the histogram) ( A ), IL-2 (JES6-5H4) ( B ) and IL-13 (13A) ( C ) by splenic i NKT cells was analyzed by intracellular cytokine staining (ICCS). Cells were fixed with Cytofix/Cytoperm for 10 min at either 4 °C or 37 °C as indicated. A summary graph (left) and representative data from gated i NKT cells (right) are in adjacent panels. The fluorochromes conjugated to the antibodies utilized are indicated below the histograms. ns = not statistically significant. Representative data from one of at least three independent experiments are shown.

    Journal: Scientific Reports

    Article Title: Improved Detection of Cytokines Produced by Invariant NKT Cells

    doi: 10.1038/s41598-017-16832-1

    Figure Lengend Snippet: Fixation method influenced the detection in i NKT cells of several cytokines. (A – C) C57BL/6 mice were either mock treated or injected i.v. with 1μg αGalCer and 90 min later the expression of the cytokines IL-4 (clone BVD6-24G2 or 11B11, as indicated in the histogram) ( A ), IL-2 (JES6-5H4) ( B ) and IL-13 (13A) ( C ) by splenic i NKT cells was analyzed by intracellular cytokine staining (ICCS). Cells were fixed with Cytofix/Cytoperm for 10 min at either 4 °C or 37 °C as indicated. A summary graph (left) and representative data from gated i NKT cells (right) are in adjacent panels. The fluorochromes conjugated to the antibodies utilized are indicated below the histograms. ns = not statistically significant. Representative data from one of at least three independent experiments are shown.

    Article Snippet: This was seen with both of the αIL-4-antibody clones, 11B11 and BVD6-24G2, that were tested (Fig. ).

    Techniques: Injection, Expressing, Staining